diff --git a/source/blender/blenkernel/intern/asset_library_service.cc b/source/blender/blenkernel/intern/asset_library_service.cc
index 14604def0d3..53ce2826022 100644
--- a/source/blender/blenkernel/intern/asset_library_service.cc
+++ b/source/blender/blenkernel/intern/asset_library_service.cc
@@ -24,6 +24,7 @@
 #include "BKE_blender.h"
 #include "BKE_callbacks.h"
 
+#include "BLI_path_util.h"
 #include "BLI_string_ref.hh"
 
 #include "MEM_guardedalloc.h"
@@ -54,14 +55,27 @@ void AssetLibraryService::destroy()
   instance_.reset();
 }
 
+namespace {
+std::string normalize_directory_path(StringRefNull directory)
+{
+
+  char dir_normalized[PATH_MAX];
+  STRNCPY(dir_normalized, directory.c_str());
+  BLI_path_normalize_dir(NULL, dir_normalized);
+  return std::string(dir_normalized);
+}
+}  // namespace
+
 AssetLibrary *AssetLibraryService::get_asset_library_on_disk(StringRefNull top_level_directory)
 {
   BLI_assert_msg(!top_level_directory.is_empty(),
                  "top level directory must be given for on-disk asset library");
 
-  AssetLibraryPtr *lib_uptr_ptr = on_disk_libraries_.lookup_ptr(top_level_directory);
+  std::string top_dir_trailing_slash = normalize_directory_path(top_level_directory);
+
+  AssetLibraryPtr *lib_uptr_ptr = on_disk_libraries_.lookup_ptr(top_dir_trailing_slash);
   if (lib_uptr_ptr != nullptr) {
-    CLOG_INFO(&LOG, 2, "get \"%s\" (cached)", top_level_directory.c_str());
+    CLOG_INFO(&LOG, 2, "get \"%s\" (cached)", top_dir_trailing_slash.c_str());
     return lib_uptr_ptr->get();
   }
 
@@ -69,10 +83,10 @@ AssetLibrary *AssetLibraryService::get_asset_library_on_disk(StringRefNull top_l
   AssetLibrary *lib = lib_uptr.get();
 
   lib->on_save_handler_register();
-  lib->load(top_level_directory);
+  lib->load(top_dir_trailing_slash);
 
-  on_disk_libraries_.add_new(top_level_directory, std::move(lib_uptr));
-  CLOG_INFO(&LOG, 2, "get \"%s\" (loaded)", top_level_directory.c_str());
+  on_disk_libraries_.add_new(top_dir_trailing_slash, std::move(lib_uptr));
+  CLOG_INFO(&LOG, 2, "get \"%s\" (loaded)", top_dir_trailing_slash.c_str());
   return lib;
 }
 
diff --git a/source/blender/blenkernel/intern/asset_library_service_test.cc b/source/blender/blenkernel/intern/asset_library_service_test.cc
index b4bb1dc0895..ed299028c41 100644
--- a/source/blender/blenkernel/intern/asset_library_service_test.cc
+++ b/source/blender/blenkernel/intern/asset_library_service_test.cc
@@ -19,6 +19,8 @@
 
 #include "asset_library_service.hh"
 
+#include "BLI_path_util.h"
+
 #include "CLG_log.h"
 
 #include "testing/testing.h"
@@ -84,6 +86,30 @@ TEST_F(AssetLibraryServiceTest, library_pointers)
    * cannot be reliably tested by just pointer comparison, though. */
 }
 
+TEST_F(AssetLibraryServiceTest, library_path_trailing_slashes)
+{
+  AssetLibraryService *service = AssetLibraryService::get();
+
+  char asset_lib_no_slash[PATH_MAX];
+  char asset_lib_with_slash[PATH_MAX];
+  STRNCPY(asset_lib_no_slash, asset_library_root_.c_str());
+  STRNCPY(asset_lib_with_slash, asset_library_root_.c_str());
+
+  /* Ensure #asset_lib_no_slash has no trailing slash, regardless of what was passed on the CLI to
+   * the unit test. */
+  while (strlen(asset_lib_no_slash) &&
+         ELEM(asset_lib_no_slash[strlen(asset_lib_no_slash) - 1], SEP, ALTSEP)) {
+    asset_lib_no_slash[strlen(asset_lib_no_slash) - 1] = '\0';
+  }
+
+  BLI_path_slash_ensure(asset_lib_with_slash);
+
+  AssetLibrary *const lib_no_slash = service->get_asset_library_on_disk(asset_lib_no_slash);
+
+  EXPECT_EQ(lib_no_slash, service->get_asset_library_on_disk(asset_lib_with_slash))
+      << "With or without trailing slash shouldn't matter.";
+}
+
 TEST_F(AssetLibraryServiceTest, catalogs_loaded)
 {
   AssetLibraryService *const service = AssetLibraryService::get();